Journal: International Journal of Molecular Sciences

Article Title: HDAC Class I Inhibitor Domatinostat Induces Apoptosis Preferentially in Glioma Stem Cells Through p53-Dependent and -Independent Activation of BAX Expression

doi: 10.3390/ijms26167803

Figure Lengend Snippet: Domatinostat preferentially inhibits cell growth and induces cell death in glioma stem cells. ( A ) GS-Y01, GS-Y03, TGS01, their differentiated counterparts (dGS-Y01, dGS-Y03, and dTGS01), and IMR-90 human lung fibroblasts were treated with the indicated concentrations of domatinostat for 1–4 days, and the number of viable cells was assessed by trypan blue dye exclusion. ( B ) Cells were treated as in ( A ), and the percentage of dead cells was evaluated using the trypan blue dye exclusion assay. * p < 0.05 vs. treatment without domatinostat (i.e., at 0 nM).

Article Snippet: IMR-90, a human normal fetal lung fibroblast cell line, was purchased from the American Type Culture Collection (Manassas, VA, USA) and maintained in DMEM supplemented with 10% FBS.

Techniques: Exclusion Assay

Journal: International Journal of Molecular Sciences

Article Title: HDAC Class I Inhibitor Domatinostat Induces Apoptosis Preferentially in Glioma Stem Cells Through p53-Dependent and -Independent Activation of BAX Expression

doi: 10.3390/ijms26167803

Figure Lengend Snippet: Domatinostat induces apoptotic cell death. ( A ) GS-Y01, GS-Y03, TGS01, their differentiated counterparts (dGS-Y01, dGS-Y03, and dTGS01), and IMR-90 human lung fibroblasts were treated without or with 500 nM domatinostat for 1–3 days, and were then subjected to Western blot analyses of the indicated proteins. The numbers below the images represent the means ( n = 2) of the relative band intensities after each band was quantified by densitometry and normalized to the GAPDH value. * p < 0.05 vs. cells before domatinostat treatment (i.e., cells at 0 days) for each of the glioma stem cell lines and their differentiated counterparts, except for the right most panel where comparisons were made with the adjacent left lane (i.e., IMR-90 treated with domatinostat for 3 days). ( B ) Cells were treated without or with 500 nM domatinostat in the absence or presence of 40 μM Z-VAD-fmk for 1 day, and were then subjected to Western blot analyses of the indicated proteins. * p < 0.05 vs. cells treated with domatinostat alone. ( C ) Cells were treated as in ( B ), and were then subjected to the propidium iodide (PI) incorporation assay to assess the percentage of dead cells. * p < 0.05. In ( A , B ), the expected migrating position for cleaved caspase-3 is indicated by an arrow.

Article Snippet: IMR-90, a human normal fetal lung fibroblast cell line, was purchased from the American Type Culture Collection (Manassas, VA, USA) and maintained in DMEM supplemented with 10% FBS.

Techniques: Western Blot